ʻO ka Novel Coronavirus (2019-nCoV) Ribonucleic Acid Detection Kit (PCR-manawa maoli - Fluorescent Probe Assay)
Manaʻo ʻiaHoʻohana
Kuhi ʻia kēia huahana no ka ʻike qualitative o ka coronavirus novel (SARS-CoV-2) i nā laʻana hanu.Hiki ke hoʻohana ʻia nā hopena ʻike e kōkua i ka maʻi lapaʻau o ka maʻi Coronavirus 2019 (COVID-19).
Nā ʻāpana
2019-nCoV PCR Buffer - 96 mau ho'āʻo
PCR Enzyme Mix - 96 mau ho'āʻo
2019-nCoV Positive Control - 48 mau ho'āʻo
2019-nCoV Negative Control - 48 mau ho'āʻo
2019-nCoV Ka Mana Kuloko - 96 mau ho'āʻo
Hoʻokomo pūʻolo - 1 kope
HuahanaMechanism
Aia i loko o kēia huahana nā ʻehā o nā PCR primers, ʻehā fluorescent probes, RNA reverse transcriptase, DNA polymerase, dNTP, magnesium ion, a me nā mea ʻē aʻe no ka ʻike RNA viral.Kuhi ia i ka RNA-dependent RNA polymerase (RdRp) gene, Nucleocapsid (N) gene, a me Envelop (E) gene o 2019-nCoV i ka manawa like i loko o hoʻokahi pahu hoʻāʻo hoʻokahi.E holo ana ke kaʻina hana hoʻāʻo ma kēia mau ʻanuʻu ʻekolu: 1) Hoʻohuli hou i ka RNA viral i loko o ka DNA viral.2) E hoʻonui i ka DNA viral i ka nui hiki ke ana.3) E hōʻike i ka nui o nā amplicons DNA ma o ka hoʻohālikelike ʻana.Ma waho aʻe o kēia huahana, loaʻa pū kekahi mana kūloko a me waho e nānā i kāna hana i ka wā o ka lawe ʻana a me ka mālama ʻana.
