Manaʻo ʻiaHoʻohana
Kūpono kēia huahana no ka ʻike qualitative o COVID-19.Hāʻawi ia i kahi kōkua i ka ʻike ʻana i ka maʻi me ka coronavirus novel.
HOOLAHA
Aia nā coronaviruses hou i ka genus β.ʻO COVID-19 kahi maʻi maʻi ʻeha hanu.Hiki ke maʻalahi nā kānaka.I kēia manawa, ʻo nā maʻi i loaʻa i ka coronavirus novel ke kumu nui o ka maʻi;Hiki i nā kānaka maʻi asymptomatic ke lilo i kumu maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1 a 14 mau lā, ʻo ka hapa nui he 3 a 7 mau lā.ʻO nā hōʻike nui e pili ana i ke kuni, ka luhi a me ka ʻū maloʻo.Loaʻa ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka maʻi maʻi i kekahi mau hihia.
KUMU
Aia i loko o ka pahu ho'āʻo ʻelua mau ʻāpana hoʻāʻo:
I loko o kekahi o ia mau mea, he pad conjugate kala burgundy i loaʻa i ka Novel coronavirus recombinant envelop antigens i hui pū ʻia me ke gula Colloid (Novel coronavirus conjugates), 2) kahi ʻāpana membrane nitrocellulose i loaʻa nā laina hoʻāʻo ʻelua (laina IgG a me IgM) a me kahi laina mana (laina C1). .
Ua uhi mua ʻia ka laina IgM me ka antibody IgM antibody iole, ua uhi ʻia ka laina IgG me ka antibody IgG anti-Human Mouse.Ke hāʻawi ʻia ka nui kūpono o ka mea hoʻāʻo i loko o ka pūnāwai laʻana o ka mea hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka hāmeʻa.ʻO ka IgM anti-Novel coronavirus, inā aia i ka specimen, e hoʻopaʻa i nā conjugates Novel coronavirus.
A laila hopu ʻia ka immunocomplex e ka reagent i uhi mua ʻia ma ka band IgM, e hana ana i kahi laina IgM kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi o ka Novel coronavirus IgM.ʻO ka IgG anti-Novel coronavirus inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Novel coronavirus.A laila hopu ʻia ka immunocomplex e ka reagent i uhi ʻia ma ka laina IgG, e hana ana i kahi laina IgG kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi o ka Novel coronavirus IgG.ʻO ka loaʻa ʻole o nā laina T (IgG a me IgM) hōʻike i kahi hopena maikaʻi ʻole.No ka lawelawe ʻana ma ke ʻano he mana kaʻina hana, e ʻike mau ʻia kahi laina kala ma ka ʻāpana laina mana e hōʻike ana ua hoʻohui ʻia ka nui kūpono o ka specimen a ua hana ʻia ka membrane wicking.
Ma ka ʻāpana ʻē aʻe, ua haku ʻia ka pahu hoʻāʻo i nā ʻāpana penei: ʻo ia hoʻi ka pad sample, pad reagent, membrane reaction, a me ka pad absorbing.Aia ka reagent pad i ka colloidal-gold conjugated me nā antibodies monoclonal e kūʻē i ka protein nucleocapsid o SARS-CoV-2;Aia ka membrane hopena i nā antibodies lua no ka protein nucleocapsid o SARS-CoV-2.Hoʻopaʻa ʻia ke kaula holoʻokoʻa i loko o kahi mea plastik.Ke hoʻokomo ʻia ka hāpana i loko o ka pūnāwai, hoʻoheheʻe ʻia nā conjugates i hoʻomaloʻo ʻia i ka papa reagent a neʻe pū me ka hāpana.Inā hōʻike ʻia ka antigen SARS-CoV-2 i ka laʻana, kahi paʻakikī i hoʻokumu ʻia ma waena o ka conjugate anti-SARS-2 a e hopu ʻia ka maʻi e nā antibodies monoclonal anti-SARS-2 kūikawā i uhi ʻia ma ka ʻāpana laina hōʻike (T).ʻO ka nele o ka laina T e hōʻike ana i kahi hopena maikaʻi ʻole.No ka lawelawe ʻana ma ke ʻano he kaʻina hana, e ʻike mau ʻia kahi laina ʻulaʻula ma ka ʻāpana laina mana (C2) e hōʻike ana ua hoʻohui ʻia ka leo kūpono o ka laʻana a ua hana ʻia ka wili ʻana o ka membrane.
Ka manawa kau: Apr-01-2021